Antimicrobial study of in-vitro generated Withania somnifera leaf extract

Moharana T
Department of Botany, Brahmani Degree College, Kendrapara, Odisha, India
Bahira S.K
Department of Botany, Palsama Science Degree College, Deogarh, Odisha, India.
Sahoo R.R
Department of Botany, Kendrapara Residential College, Kendrapara, Odisha, India.
Tejashweta S
Department of Biology, Samanta Chandrasekhar Institute of Technology and Management, Koraput, Odisha, India.


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The objective of this work is to study the antimicrobial effect of in-vitro grown leaf extracts of Withania somnifera (L.) against some selected known microorganisms and the use of its constituents present there for the preparation of therapeutic compounds. This is evaluated by using zone of inhibition studies and minimum inhibitory concentration. The microorganisms used include Shigella flexneri, Salmonella typhimurium, Vibrio cholera and Candida parapsilosis. Inhibition zones of plant extracts were compared with standard antibiotics like Neomycin, and Kanamycin. Inhibition zones are revealed by methanol extract which is comparatively more than the ethanol extract.  For methanol extract, Salmonella typhimurium shows the highest inhibition zone (25.13±0.52) whereas Vibrio cholera shows the least inhibition zone (21.33±0.53). The same extract shows maximum antimicrobial activity against Salmonella typhimurium followed by Candida parapsilosis and Shigella flexneri. These possibilities can build-up a novel idea in the preparation of pharmaceutical products.


  • Withania somnifera,
  • Antimicrobial activity,
  • Leaf extract,
  • Ayurvedic medicine,
  • Pharmaceutical product


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Article Details

Volume 3, Issue 1, Year 2021

Published 2021-06-02


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How to Cite

T, M., S.K, B., R.R, S., & S, T. (2021). Antimicrobial study of in-vitro generated Withania somnifera leaf extract. Bulletin of Scientific Research, 3(1), 59–65.